Asbestos Abatement >> How To Dispose Of Asbestos Waste In New Jersey

Send the samples to the laboratory with paperwork requesting asbestos analysis. List any known fibrous interferences present during sampling on the paperwork. Also, note the workplace operation(s) sampled. 5.3.2.Secure and handle the samples in such that they will not rattle during How To Dispose Of Asbestos Waste In New Jersey shipment nor be exposed to static electricity. 

Do not ship samples in expanded polystyrene peanuts, vermiculite, paper shreds, or excelsior. Tape sample cassettes to sheet bubbles and place in a container that will cushion the How To Dispose Of Asbestos Waste In New Jersey samples in such a manner that they will not rattle. 5.3.3.To avoid the possibility of sample contamination, always ship bulk samples in separate mailing containers. 

Analysis 6.1. Safety Precautions 6.1.1.Acetone is extremely flammable and precautions must be taken not to ignite it. Avoid using large containers or quantities of acetone. Transfer the solvent in a ventilated laboratory hood. Do not use acetone near any open flame. For How To Dispose Of Asbestos Waste In New Jersey generation of acetone vapor, use a spark free heat source. 

Any asbestos spills should be cleaned up immediately to prevent dispersal of fibers. Prudence should be exercised to avoid contamination of laboratory facilities or exposure of How To Dispose Of Asbestos Waste In New Jersey personnel to asbestos. Asbestos spills should be cleaned up with wet methods and/ or a High Efficiency Particulate-Air (HEPA) filtered vacuum. 

Caution: Do not use a How To Dispose Of Asbestos Waste In New Jerseyvacuum without a HEPA filter -- It will disperse fine asbestos fibers in the air. 6.2. Equipment 6.2.1. Phase contrast microscope with binocular or trinocular head. 6.2.2. Widefield or Huygenian 10X eyepieces (Note: The eyepiece containing the graticule must be a focusing eyepiece. 

Use a 40X phase objective with a numerical aperture of 0.65 to 0.75). 6.2.3. Kohler illumination (if possible) with green or blue filter. 6.2.4. Walton-Beckett Graticule, type G-22 with 100 plus or minus 2 um projected diameter. 6.2.5. Mechanical stage. A rotating mechanical How To Dispose Of Asbestos Waste In New Jersey stage is convenient for use with polarized light. 

Phase telescope. 6.2.7. Stage micrometer with 0.01-mm subdivisions. 6.2.8. Phase-shift test slide, mark II (Available from PTR optics Ltd., and also  How To Dispose Of Asbestos Waste In New Jersey McCrone). 6.2.9. Precleaned glass slides, 25 mm X 75 mm. One end can be frosted for convenience in writing sample numbers, etc., or paste-on labels can be used. 6.2.10. 

Cover glass #1 1/2. 6.2.11. Scalpel (#10, curved blade). 6.2.12. Fine tipped forceps. 6.2.13. Aluminum block for clearing filter (see Appendix D and  How To Dispose Of Asbestos Waste In New Jersey Figure 4). 6.2.14. Automatic adjustable pipette, 100- to 500-uL. 6.2.15. Micropipette, 5 uL. 6.3. Reagents 6.3.1. Acetone (HPLC grade). 6.3.2. Triacetin (glycerol triacetate). 

Lacquer or nail polish. 6.4. Standard Preparation A way to prepare standard asbestos samples of known concentration has not been developed. It is possible to prepare replicate How To Dispose Of Asbestos Waste In New Jersey samples of nearly equal concentration. This has been performed through the PAT program. These asbestos samples are distributed by the AIHA to participating laboratories. 

Since only about one-fourth of a 25-mm sample membrane is required for an asbestos count, any PAT sample can serve as a "standard" for replicate counting. 6.5. Sample Mounting Note: See Safety Precautions in How To Dispose Of Asbestos Waste In New Jersey Section 6.1. before proceeding. 

The objective is to produce samples with a smooth (non-grainy) background in a medium with a refractive index of approximately 1.46. The technique below collapses the filter for easier focusing and produces How To Dispose Of Asbestos Waste In New Jersey permanent mounts which are useful for quality control and inter laboratory comparison. An aluminum block or similar device is required for sample preparation. 

Heat the aluminum block to about 70 deg. C. The hot block should not be used on any surface that can be damaged by either the heat or from How To Dispose Of Asbestos Waste In New Jersey exposure to acetone. 6.5.2. Ensure that the glass slides and cover glasses are free of dust and fibers. 6.5.3. Remove the top plug to prevent a vacuum when the cassette is opened. 

Clean the outside of the cassette if necessary. Cut the seal and/or tape on the cassette with a razor blade. Very carefully separate the How To Dispose Of Asbestos Waste In New Jersey base from the extension cowl, leaving the filter and backup pad in the base. 6.5.4. With a rocking motion cut a triangular wedge from the filter using the scalpel. This wedge should be one-sixth to one-fourth of the filter. 

Grasp the filter wedge with the forceps on the perimeter of the filter which was clamped between the cassette pieces. DO NOT TOUCH the filter with your finger. Place the filter on the glass slide How To Dispose Of Asbestos Waste In New Jersey sample side up. Static electricity will usually keep the filter on the slide until it is cleared. 6.5.5. Place the tip of the micropipette containing about 200 uL acetone into the aluminum block. 

Insert the glass slide into the receiving slot in the aluminum block. Inject the acetone into the block with slow, steady pressure on the plunger while How To Dispose Of Asbestos Waste In New Jersey holding the pipette firmly in place. Wait 3 to 5 seconds for the filter to clear, then remove the pipette and slide from the aluminum block. 

Immediately (less than 30 seconds) place 2.5 to 3.5 uL of triacetin on the filter (Note: Waiting longer than 30 seconds will result in increased index of refraction and decreased contrast between the fibers and the How To Dispose Of Asbestos Waste In New Jersey preparation. This may also lead to separation of the cover slip from the slide).  

Lower a cover slip gently onto the filter at a slight angle to reduce the possibility of forming air bubbles. If more than 30 seconds have elapsed between acetone exposure and triacetin How To Dispose Of Asbestos Waste In New Jersey application, glue the edges of the cover slip to the slide with lacquer or nail polish.  

If clearing is slow, warm the slide for 15 min on a hot plate having a surface temperature of about 50 deg. C to hasten clearing. The top of the hot block can be used if the slide is not heated too long. Counting may proceed immediately after clearing and mounting are completed. 6.6. Sample How To Dispose Of Asbestos Waste In New Jersey Analysis 

Completely align the microscope according to the manufacturer's How To Dispose Of Asbestos Waste In New Jersey instructions. Then, align the microscope using the following general alignment routine at the beginning of every counting session and more often if necessary. 6.6.1. Alignment (1) Clean all optical surfaces. Even a small amount of dirt can significantly degrade the image. 

Rough focus the objective on a sample. (3) Close down the field iris so that it is visible in the field of view. Focus the How To Dispose Of Asbestos Waste In New Jersey image of the iris with the condenser focus. Center the image of the iris in the field of view. (4) Install the phase telescope and focus on the  phase rings. Critically center the rings. 

Misalignment of the rings results in astigmatism which will  degrade the image. (5) Place the phase-shift test slide on the microscope stage and focus on the lines. The How To Dispose Of Asbestos Waste In New Jersey analyst must see line set 3 and should see at least parts of 4 and 5 but, not see line set 6 or 6. A microscope/microscopist combination which does not pass this test may not be used.

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